Sürdürülebilir Kalkınma Amaçları

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• MakaleAçık Erişim2022/r/kostu/10807
  Biological Activities and Chemical Composition of Turkish Sweetgum Balsam (Styrax Liquidus) Essential Oil

  Büyükkiliç Altinbasak, Betul; Issa, Ghassan; Zengin Kurt, Belma; Demirci, Betul

  Objective: The purpose of this present study was to make a chemical analysis of the composition of essential oil obtained from sweetgum balsam and to examine its antimicrobial, anticholinesterase, alpha-glucosidase inhibition, and antioxidant activities.Methods: The essential oil obtained by the hydrodistillation method was analyzed by gas chromatography-mass spectrometry systems. The antimicrobial activities of the essential oil were evaluated by disc diffusion and resazurin microplate methods, the anticholinesterase effect was determined by using in vitro AChE and BChE enzymes inhibition assays, and the antioxidant effect was evaluated by ABTS and CUPRAC methods.Results: The main components of the essential oil were determined as styrene (92.6%) and alpha-pinene (2.2%). The essential oil showed weak antimicrobial activity against K. pneumoniae, S. epidermidis but strong antimicrobial activity against A. baumannii, C. glabrata. It showed moderate inhibitory activity to AChE and BChE enzymes, and IC50 values were calculated as 36.5 mu g/mL and 69.5 mu g/mL, respectively. It also showed low inhibition of alpha-glucosidase (IC50 value was 637.2 mu g/mL) and a similar antioxidant effect in the CUPRAC and ABTS method (A0.5 value was 637.2 mu g/mL and IC50 value was 632.2 mu g/mL, respectively).Conclusion: Styrax Liquidus essential oil can be considered a natural antimicrobial agent due to its strong antimicrobial activity capacity against A. baumannii and C. glabrata strains.

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  AtıfWoS5·OpenAlex5·TR Dizin2

  İncele
• MakaleAçık Erişim2022/r/kostu/10804
  Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey

  Büyükkiliç Altinbasak, Betul; Ecevit Genç, Gulay; Zengin Kurt, Belma; Demirci, Betul

  Objective: This present study was conducted to determine the interspecific chemical variability and evaluate the biological effects of the essential oils of Daucus L. species growing naturally in Turkey. The species were D. carota, D. broteri, D. guttatus, D. littoralis, D. involucratus and D. conchitae (endemic).Methods: The essential oils were obtained from fruit samples by distillation method and were analyzed both by GC-FID and GC-MS. The anti-inflammatory and anticholinesterase effects of the essential oils were investigated. The anti-inflammatory effect was evaluated by in vitro LOX enzyme inhibition activity. The anticholinesterase effect was tested on AChE and BChE enzymes. Results: The components, ratios, and yields of D. carota essential oils differed depending on the locations where the samples were collected. The main components were detected as carotol (1-74.6%), 13-bisabolen (0.9-62.4%), 11 alpha H-himachal-4-en-113- ol (0.3-49.4%), trans-methylisoeugenol (1-45.7%). The main volatile compounds were found in D. broteri, D. guttatus, D. involucratus, D. littoralis, D. conchitae as 13-sinensal (30.4%), methyleugenol (30.5%), methyleugenol (40.9%), alpha-humulene (29.4%), methyleugenol (29.6%) respectively. The essential oils didn't exhibit anti-inflammatory activity. Two essential oil samples of D. carota showed high anticholinesterase effects compared to the standard. The AChE IC50 was calculated as 6.04 +/- 0.30 mu g/mL, 2.15 +/- 0.10 mu g/mL (Galantamine IC50 1.13 +/- 0.02 mu g/mL) and BChEIC50 11.32 +/- 0.20 mu g/mL, 31.03 +/- 0.02 mu g/mL (Galantamine IC50 12.15 +/- 0.36 mu g/mL). These essential oils contained high levels of 11 alpha H-himachal-4-en-113-ol (25.04%, 49.42%).Conclusion: Because of their anticholinesterase potential, some D. carote essential oils can be evaluated in the preparation of pharmaceutical or nutraceutical products as a complementary therapy for Alzheimer's disease by standardizing their components.

  AtıfWoS4·OpenAlex4

  İncele
• MakaleAçık Erişim2022/r/kostu/10786
  Bioactive Components and Antioxidant and Antimicrobial Activities of Rhus coriaria, a Sumac Species found in Turkey

  Çaliskan, Reyhan; Sari, Silva Polat; Altinbasak, Betul Buyukkilic; Dinç, Harika Oyku; Balekoglu, Aleyna; Issa, Ghassan; Mayda, Pelin Yuksel

  Objective: In our study, it was aimed to analyze the antioxidant and antimicrobial activities of the extracts of the fruits of Rhus coriaria L. (sumac) species collected from Gaziantep.Methods: Ethanol extracts of 80% (R2) and 100% (R3) were prepared from Rhus coriaria fruits. Chemical analysis of the extracts were performed by liquid chromatography-high resolution mass spectrometry method, their antioxidant activities were investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, and their antimicrobial activity was investigated using broth microdilution method.Results: In the chemical analysis of R2 and R3 extracts, fumaric acid, an organic acid with the highest concentration, was found at concentrations of 31076.55 and 23348.37 mg/kg, respectively. While the phenolic components with the highest concentration in R2 were observed as hyperoside (622.24 mg/kg), ellagic acid (343.63 mg/kg) and p-coumaric acid (182.91 mg/kg), the phenolic components with the highest concentration in R3 were observed as ellagic acid (607.30 mg/kg), hyperoside (440.41 mg/kg) and p-coumaric acid (178.61 mg/kg). In antioxidant activities of R2 and R3 extracts, DPPH free radical scavenging activities were found to be 70.78%+/- 0.002% and 11.19 +/- 0.001%, respectively. Antimicrobial activities of R2 and R3 extracts were found to be 125 and <3.9 mu g/ mL in S. aureus strain ATCC 25923, 15.625 and 31.25 mu g/mL in A. baumannii strain ATCC 19606, 62.5 mu g/mL in H. pylori strain ATCC 43504, 62.5 mu g/mL in C. glabrata strain ATCC 2001, <3.9 mu g/mL in C. albicans strain ATCC 66027, respectively.Conclusion: The higher antioxidant activity in the R2 extract obtained from R. coriaria fruits grown in our country may be due to the higher phenolic component content compared to the R3 extract. It is thought that the more effective antimicrobial activity detected in the R3 extract may be due to the higher amount of ellagic acid compared to the R2 extract.

  AtıfWoS6·TR Dizin2

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