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Yayınlar
- MakaleAçık Erişim2022Biological Activities and Chemical Composition of Turkish Sweetgum Balsam (Styrax Liquidus) Essential Oil
Büyükkiliç Altinbasak, Betul; Issa, Ghassan; Zengin Kurt, Belma; Demirci, Betul
Objective: The purpose of this present study was to make a chemical analysis of the composition of essential oil obtained from sweetgum balsam and to examine its antimicrobial, anticholinesterase, alpha-glucosidase inhibition, and antioxidant activities.Methods: The essential oil obtained by the hydrodistillation method was analyzed by gas chromatography-mass spectrometry systems. The antimicrobial activities of the essential oil were evaluated by disc diffusion and resazurin microplate methods, the anticholinesterase effect was determined by using in vitro AChE and BChE enzymes inhibition assays, and the antioxidant effect was evaluated by ABTS and CUPRAC methods.Results: The main components of the essential oil were determined as styrene (92.6%) and alpha-pinene (2.2%). The essential oil showed weak antimicrobial activity against K. pneumoniae, S. epidermidis but strong antimicrobial activity against A. baumannii, C. glabrata. It showed moderate inhibitory activity to AChE and BChE enzymes, and IC50 values were calculated as 36.5 mu g/mL and 69.5 mu g/mL, respectively. It also showed low inhibition of alpha-glucosidase (IC50 value was 637.2 mu g/mL) and a similar antioxidant effect in the CUPRAC and ABTS method (A0.5 value was 637.2 mu g/mL and IC50 value was 632.2 mu g/mL, respectively).Conclusion: Styrax Liquidus essential oil can be considered a natural antimicrobial agent due to its strong antimicrobial activity capacity against A. baumannii and C. glabrata strains.
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İncele - MakaleAçık Erişim2022Anticholinesterase and Anti-inflammatory Activities of Essential Oils of Naturally Grown Daucus L. Species in Turkey
Büyükkiliç Altinbasak, Betul; Ecevit Genç, Gulay; Zengin Kurt, Belma; Demirci, Betul
Objective: This present study was conducted to determine the interspecific chemical variability and evaluate the biological effects of the essential oils of Daucus L. species growing naturally in Turkey. The species were D. carota, D. broteri, D. guttatus, D. littoralis, D. involucratus and D. conchitae (endemic).Methods: The essential oils were obtained from fruit samples by distillation method and were analyzed both by GC-FID and GC-MS. The anti-inflammatory and anticholinesterase effects of the essential oils were investigated. The anti-inflammatory effect was evaluated by in vitro LOX enzyme inhibition activity. The anticholinesterase effect was tested on AChE and BChE enzymes. Results: The components, ratios, and yields of D. carota essential oils differed depending on the locations where the samples were collected. The main components were detected as carotol (1-74.6%), 13-bisabolen (0.9-62.4%), 11 alpha H-himachal-4-en-113- ol (0.3-49.4%), trans-methylisoeugenol (1-45.7%). The main volatile compounds were found in D. broteri, D. guttatus, D. involucratus, D. littoralis, D. conchitae as 13-sinensal (30.4%), methyleugenol (30.5%), methyleugenol (40.9%), alpha-humulene (29.4%), methyleugenol (29.6%) respectively. The essential oils didn't exhibit anti-inflammatory activity. Two essential oil samples of D. carota showed high anticholinesterase effects compared to the standard. The AChE IC50 was calculated as 6.04 +/- 0.30 mu g/mL, 2.15 +/- 0.10 mu g/mL (Galantamine IC50 1.13 +/- 0.02 mu g/mL) and BChEIC50 11.32 +/- 0.20 mu g/mL, 31.03 +/- 0.02 mu g/mL (Galantamine IC50 12.15 +/- 0.36 mu g/mL). These essential oils contained high levels of 11 alpha H-himachal-4-en-113-ol (25.04%, 49.42%).Conclusion: Because of their anticholinesterase potential, some D. carote essential oils can be evaluated in the preparation of pharmaceutical or nutraceutical products as a complementary therapy for Alzheimer's disease by standardizing their components.
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