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- MakaleYalnız künye2024Effect of chronic stress on serum and tissue levels of klotho and tnf-α
Acar, Esra; Colak, Tuncay; Eraldemir, Fatma Ceyla; Yener, Mehmet Deniz
Objective: Chronic stress is recognized as a factor that affects almost all organs and tissues and disrupts homeostasis. Stress also directly impacts the inflammatory process. In addition, klotho is a protein associated with lifespan. This study investigates the relationship that chronic stress has with klotho and tumor necrosis factor alpha (TNF-α), which is a key protein in the proinflammatory process. Materials and Methods: The study involves 16 Wistar albino rats divided into control and stress groups. The study applies the protocol for the chronic unpredictable mild stress (CUMS) model and uses the enzymelinked immunosorbent assay (ELISA) method to measure the TNF-α and Klotho levels in the serum, heart, aorta, and liver tissues. Results: The serum samples obtained from the stress group were found to have a significant decrease in klotho levels (p=0.013) and high TNF-α levels (p=0.042). However, no significant difference was observed in the TNF-α or klotho levels in the heart, aorta, and liver tissues between groups. Conclusion: Under chronic stress, a significant increase is observed in TNF- α, one of the main cytokines of inflammation, as well as a decrease in klotho hormone. This research endeavors to contribute to the existing body of knowledge concerning the interplay among stress, inflammation, and longevity.
- MakaleAçık Erişim2023Effect of Chronic Unpredictable Mild Stress on Hippocampus and Serum Markers in Rats
Yener, Mehmet Deniz; Colak, Tuncay; Ozbek, Sema Kurnaz; Ceylan, Sureyya; Acar, Esra; Kir, Hale Maral
Objective: Unpredictable stress is a common factor that we encounter in our daily lives. In this context, we wanted to investigate the morphological effects of a chronic unpredictable mild stress (CUMS) model on rat hippocampus and serum levels of brain derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP). Materials and Methods: This study was carried out on 16 Wistar albino rats which were divided into control and experiment groups. The CUMS model protocol was applied, and the morphological structures of the hippocampus were examined. The serum GFAP/BDNF levels were measured by ELISA method. Results: The mean BDNF level was 1.65 ± 0.17 ng/mL in the control group and 2.25 ± 0.29 ng/mL in the CUMS group. The mean GFAP level was 3.04 ± 0.45 ng/mL in the control group and 2.96 ± 0.58 ng/mL in the CUMS group. When the polymorph cell layer (PCL) in gyrus dentatus was examined; the mean number of cells in the control group was 20 ± 1.38 for the left PCL and 17 ± 1.35 for the right PCL. In the stress group; the mean number of cells in the polymorph cell layer of gyrus dentatus was 27 ± 2.08 cells for the left side and 23 ± 2.01 for the right side. Conclusions: As a result, morphological changes were observed in the gyrus dentatus region which has an important role in memory formation. The cells in the polymorph cell layer of gyrus dentatus underwent changes under stress.
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